John Calvin Commentary John 16:32

"Behold, the hour cometh, yea, is come, that ye shall be scattered, every man to his own, and shall leave me alone: and [yet] I am not alone, because the Father is with me." — John 16:32 (ASV)

Yet I am not alone. This correction is added to inform us that, when Christ is forsaken by people, He loses nothing of His dignity. For since His truth and glory are founded on Himself and do not depend on what the world believes, if it happens that He is forsaken by the whole world, He is still in no way impaired, because He is God and does not need any assistance from another.

Because my Father is with me. When He says that the Father will be with me, the meaning is that God will be on His side, so that He will have no need to borrow anything from people. Whoever meditates on this in a proper manner will remain firm, even if the whole world is shaken, and the revolt of all people will not overturn his faith; for we do not give God the honor that is due to Him if we are not satisfied with having God alone.