John Gill Commentary Nehemiah 3:27

"After him the Tekoites repaired another portion, over against the great tower that standeth out, and unto the wall of Ophel." — Nehemiah 3:27 (ASV)

After them the Tekoites repaired another piece
Having finished what they undertook in another part of the wall, (Nehemiah 3:5) , they engage in this part of it; which shows their great zeal and diligence, when their nobles were so backward to it, and withdrew from it:

over against the great tower that lieth out ;
the same as in the preceding verse:

even unto the wall of Ophel ;
from right against the great tower unto the wall the Tekoites repaired.